![]() For biosensors electrosynthesized in neutral buffer under the present conditions, entrapment of the GOx within the PoPD layer produced the design (Pt C/PoPD-GOx) with the highest linear sensitivity to glucose (5.0 ± 0.4 μA cm −2 mM −1), good linear range ( K M = 16 ± 2 mM) and response time (< 2 s), and the greatest AA blocking (99.8% for 1 mM AA). ![]() A number of selectivity parameters were used to identify the most successful design in terms of the balance between substrate sensitivity and interference blocking. These electrodes were calibrated with H 2O 2 (the biosensor enzyme signal molecule), glucose, and the archetypal interference compound ascorbic acid (AA) to determine the relevant polymer permeabilities and the apparent Michaelis-Menten parameters for glucose. In an ongoing programme to develop characterization strategies relevant to biosensors for in-vivo monitoring, glucose biosensors were fabricated by immobilizing the enzyme glucose oxidase (GOx) on 125 μm diameter Pt cylinder wire electrodes (Pt C), using three different methods: before, after or during the amperometric electrosynthesis of poly( ortho-phenylenediamine), PoPD, which also served as a permselective membrane.
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